Source : |
HEK293 |
Species : |
Human |
Tag : |
His&StrepII |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Loaded Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.27 μM as determined in BLI assay (ForteBio Octet Red96e).Loaded Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag on AR2G Biosensor, can bind Herceptin with an affinity constant of 0.35 μM as determined in BLI assay (ForteBio Octet Red96e).Loaded Biotinylated Human Serum Albumin, His,Avitag on SA Biosensor, can bind Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag with an affinity constant of 0.641 μM as determined in BLI assay (ForteBio Octet Red96e). |
Molecular Mass : |
Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.2 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation. |
Protein Length : |
Ala24-Ser297(FCGRT)&Ile21-Met119(B2M) |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |