FCGRT & B2M-1927H
Recombinant Human FCGRT/B2M protein, His/Strep II-tagged, Biotinylated
Recombinant Human FCGRT(Ala 24 - Ser 297)/B2M(Ile 21 - Met 119), the subunit FcRN carries a polyhistidine tag at the C-terminus.the subunit B2M carries a Strep II tag at the C-terminus was expressed in HEK293.
Predicted N Terminal:
Ala 24(FcRN) & Ile 21(B2M)
Lyophilized from 0.22um filtered solution in PBS (pH 7.4) with Trehalose as protectant.
The protein has a calculated MW of 31.2 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 33 kDa (FCGRT), 13 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
FCGRT(Ala 24 - Ser 297)/B2M(Ile 21 - Met 119)
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.No activity loss is observed after storage at:4-8 centigrade for 12 months in lyophilized state;-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.