Recombinant mature form of human FGF10 (NP_004456.1) (Gln 38-Ser 208) was expressed.
Predicted N Terminal:
Lyophilized from sterile 20mM Tris, pH 7.5, 5%~8% trehalose and mannitol.
Measured by its binding ability in a functional ELISA.Immobilized humen FGF10 at 10 μg/ml (100 μl/well) can bind Cynomolgus FGFR1-Fc with a linear range of 31.25-2000 ng/mL.
The recombinant human FGF10 consists of 172 amino acids and has a predicted molecular mass of 19.6 kDa as estimated in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>98 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.