Recombinant human FGF14 isoform 1B (NP_787125.1) (Lys 64-Thr 252) was expressed and purified.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.5, 5%~8% trehalose and mannitol.
Measured by its ability to bind human FGFR4-Fc (Cat:10538-H02H) in a functional ELISA.
The recombinant human FGF14 consisting of 190 amino acids and has a calculated molecular mass of 21.1KDa. It migrates as an approximately 18KDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>97 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.