Active Recombinant Human FGF22 protein, His-tagged

• Cat.No.: FGF22-32H
• Product Overview: Recombinant Human FGF22(Thr23-Ser170) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 μm filtered solution in MES, Na2SO4, EDTA, Sucrose and DTT with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is typically 50-300 ng/mL.
• Molecular Mass: 18 kDa
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: FGF22 fibroblast growth factor 22 [ Homo sapiens ]
• Official Symbol: FGF22
• Synonyms: FGF22; fibroblast growth factor 22; FGF-22
• Gene ID: 27006
• mRNA Refseq: NM_020637
• Protein Refseq: NP_065688
• MIM: 605831
• UniProt ID: Q9HCT0
• Chromosome Location: 19p13.3
• Pathway: Downstream signaling of activated FGFR, organism-specific biosystem; FGFR ligand binding and activation, organism-specific biosystem; FGFR1 ligand binding and activation, organism-specific biosystem; FGFR1b ligand binding and activation, organism-specific biosystem
• Function: growth factor activity