Recombinant Human FGF22(Thr23-Ser170) fused with His tag at N-terminal was expressed in E. coli.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in MES, Na2SO4, EDTA, Sucrose and DTT with BSA as a carrier protein.
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is typically 50-300 ng/mL.
<0.01 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.