Recombinant human FGFR1 isoform 4 (NP_075594.1) cytoplasmic domain (Gly 311-Arg 731) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Predicted N Terminal:
Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 0.3mM DTT.
1. Kinase activity untested2. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR1 (aa 311-731) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF acidic with a linear range of 15.6-250 ng/ml.3. Measured by i
The recombinant human FGFR1 (aa 311-731) /GST chimera consists of 658 amino acids and has a calculated molecular mass of 75 KDa. It migrates as an approximately 75 KDa band as predicted in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>93 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.