Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Recombinant Human FLG protein, His-tagged

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By Species
Human(7)
By Source
By Tag
GST(2)
His(1)
N/A(1)
Cat.No.:
FLG-1229H
Product Overview:
Recombinant Human FLG(Ser3905~Tyr4031) fused with His tag at N-terminal was expressed in E. coli.
Description:
The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.
Source:
E. coli
Species:
Human
Tag :
His
Form:
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300
Molecular Mass:
25kDa
Protein length:
Ser3905~Tyr4031
AA Sequence:
SHRDTA SHVRSSPVQS DSSTAKEHGH FSSLSQDSAY HSGIQSRGSP
Endotoxin:
<1.0EU per 1µg (determined by the LAL method)
Purity:
> 95%
Applications:
Positive Control; Immunogen; SDS-PAGE; WB. If bio-activity of the protein is needed, please check active protein
Stability:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage:
Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution:
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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