Recombinant Human FLT3 was expressed in Insect cells.
FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
Predicted N Terminal:
The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser.
The protein was lyophilized with no additives.
The ED50, calculated by the dose-dependent stimulation of the proliferation of human MUTZ-2 cells is< 3.0 ng/ml.
155 amino acids
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution Flt3-L should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.