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Recombinant Human FLT3 protein

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Cat.No.:
FLT3-185H
Product Overview:
Recombinant Human FLT3 was expressed in Insect cells.
Description:
FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
Source:
Insect cells
Species:
Human
Predicted N Terminal:
The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser.
Form:
The protein was lyophilized with no additives.
Bio-activity:
The ED50, calculated by the dose-dependent stimulation of the proliferation of human MUTZ-2 cells is< 3.0 ng/ml.
Molecular Mass:
19 kDa
Protein length:
155 amino acids
Purity:
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Storage:
Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution Flt3-L should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Reconstitution:
It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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