Active Recombinant Human FLT3LG protein

• Cat.No.: FLT3LG-155H
• Product Overview: Recombinant Human FLT3LG(Thr27-Pro185) was expressed in Insect cells.

Specification

• Source: Insect cells
• Species: Human
• Predicted N Terminal: Thr27
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is typically 0.2-1 ng/mL.The specific activity of Recombinant Human Flt-3 Ligand is approximately 1.2 x 10^3 U/μg, which is calibrated against recombinant human Flt-3 Ligand WHO Standard.
• Molecular Mass: Predicted Molecular Mass: 17.5 kDa;SDS-PAGE: 17-30 kDa, reducing conditions.
• Protein length: Thr27-Pro185
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: FLT3LG fms-related tyrosine kinase 3 ligand [ Homo sapiens ]
• Official Symbol: FLT3LG
• Synonyms: FLT3LG; fms-related tyrosine kinase 3 ligand; flt3 ligand; FL; FLT3L
• Gene ID: 2323
• mRNA Refseq: NM_001204502
• Protein Refseq: NP_001191431
• MIM: 600007
• UniProt ID: P49771
• Chromosome Location: 19q13.3
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Pathways in cancer, organism-specific biosystem
• Function: cytokine activity; protein homodimerization activity; receptor binding; receptor tyrosine kinase binding