Recombinant Human FLT3LG protein

Cat.No.: FLT3LG-155H
Product Overview: Recombinant Human FLT3LG(Thr27-Pro185) was expressed in Insect cells.
Source: Insect cells
Species: Human
Predicted N Terminal: Thr27
Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Bio-activity: Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is typically 0.2-1 ng/mL.The specific activity of Recombinant Human Flt-3 Ligand is approximately 1.2 x 10^3 U/μg, which is calibrated against recombinant human Flt-3 Ligand WHO Standard.
Molecular Mass: Predicted Molecular Mass: 17.5 kDa;SDS-PAGE: 17-30 kDa, reducing conditions.
Protein length: Thr27-Pro185
Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Gene Name: FLT3LG fms-related tyrosine kinase 3 ligand [ Homo sapiens ]
Official Symbol: FLT3LG
Synonyms: FLT3LG; fms-related tyrosine kinase 3 ligand; flt3 ligand; FL; FLT3L;
Gene ID: 2323
mRNA Refseq: NM_001204502
Protein Refseq: NP_001191431
MIM: 600007
UniProt ID: P49771
Chromosome Location: 19q13.3
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Pathways in cancer, organism-specific biosystem;
Function: cytokine activity; protein homodimerization activity; receptor binding; receptor tyrosine kinase binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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