Recombinant Human FLT3LG(Thr27-Pro185) was expressed in Insect cells.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is typically 0.2-1 ng/mL.The specific activity of Recombinant Human Flt-3 Ligand is approximately 1.2 x 10^3 U/μg, which is calibrated against recombinant human Flt-3 Ligand WHO Standard.
Predicted Molecular Mass: 17.5 kDa;SDS-PAGE: 17-30 kDa, reducing conditions.
<0.10 EU per 1 μg of the protein by the LAL method.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.