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Home > Products > Recombinant Proteins > Recombinant Human GUK1 protein, His-tagged

Recombinant Human GUK1 protein, His-tagged

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Human(14)
Mouse(8)
Rat(3)
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GST(1)
His(5)
Cat.No.:
GUK1-185H
Product Overview:
Recombinant Human GUK1(Ser2-Ala197) fused with His tag at N-terminal was expressed in E. coli.
Description:
GUK1, also known as GMP kinase, belongs to the guanylate kinase family. This protein exists as a monomer and catalyzes the ATP-dependent conversion of GMP to GDP, thereby playing an essential role in the recycling of GMP. Via its catalytic activity, GUK1 participates in regulating the supply of guanine nucleotides including cGMP to signal transduction pathways. GUK1 is widely expressed. Overexpression of GUK1 is associated in pituitary adenomas. The enzymatic activity of the recombinant human GUK1 is measured using a phosphatase-coupled method.
Source:
E. coli
Species:
Human
Tag :
His
Predicted N Terminal:
Met
Form:
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Bio-activity:
Measured by its ability to transfer phosphate from ATP to GMP. The specific activity is >7,500 pmol/min/μg.
Molecular Mass:
Predicted Molecular Mass: : 23 kDa;
SDS-PAGE: 24 kDa, reducing conditions.
Endotoxin:
<1.0 EU per 1 μg of the protein by the LAL method.
Purity:
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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