Recombinant human Histone 3.3 (H2F3A) cDNA (136aa, which derived from BC066901) fused with T7-His-TEV cleavage site Tag (29aa) at N-terminal was expressed in E. coli.
0.25 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
MASMTGGQQMGRGHHHHHHGNLYFQGGEFARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTV ALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMP KDIQLARRIRGERA
1. May be used for in vitro Histone 3.3 mediated HIRA-dependent H3.3 deposition in cell reprogramming regulation study with "ProFectin" based intracellular delivery of this protein.2. May be used as specific substrate protein for kinase and ubiquitin (Sumo pathway) related enzyme functional screening assays.3. May be used for H3.3 protein-protein interaction mapping.4. May be used as antigen for specific antibody production.
Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.