Recombinant Human HAO1(Leu 2 - Ile 370) fused with His tag at N-terminal was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Has a calculated MW of 41.7 kDa. The reducing (R) protein migrates as 41.7 kDa in SDS-PAGE .
Leu 2 - Ile 370
Less than 1.0 EU per μg of the Human HAO-1, His Tag by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).