Human TIM-3 / HAVCR2 (22-200) Protein, Strep Tag, Ser 22 - Arg 200 (Accession # NP_116171) was produced in human 293 cells (HEK293).
Predicted N Terminal:
Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
Measured by its binding ability in a functional ELISA. Immobilized Human TIM-3 (22-200), Strep Tag at 2 μg/mL (100 ul/well),can bind ABTIM3 with a linear range of 0.2-3 ng/mL.
Human TIM-3 (22-200), Strep Tag is fused with twin strep tag at the C-terminus, and has a calculated MW of 22.9 kDa. The reducing protein migrates as 20 kDa and 30-45 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.