Recombinant Human HBG2(1-147aa) fused with His tag at N-terminal was expressed in E. coli.
Hemoglobin subunit gamma-2, also known as HBG2, belongs to the fetal hemoglobin subunit, which consists of two alpha chains together with two gamma chains. Increased fetal hemoglobin production in adults can ameliorate the clinical severity of sickle cell disease and beta-thalassemia major.
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol,1mM DTT
18.5 kDa (170aa) confirmed by MALDI-TOF
> 90% by SDS - PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.5 mg/ml (determined by Bradford assay)