Recombinant Human HIST1H3A was expressed in E. coli.
A nuclesome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around a histone octamer of two copies of each of the core histones, H2A, H2B, H3, and H4. The combination of two H2A/H2B dimers and one H3/H4 tetramer creat the nucleosome core. Histone H3 undergoes many modifications which include acetylation, methylation, an phosphorylation that are important for regulation of gene transcription.
> 95% as determined by SDS-PAGE
50 mM sodium phosphate, PH 7.2, containing 100 mM sodium chloride, and 20% glycerol.