Recombinant Human HIST3H3 was expressed in E. coli.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Recombinant histone H3 is suitable for enzyme assays and nucleosome reconstitution.
Lyophilized proteins are stable for 2 years at -20 centigrade from date of shipment. Reconstitute with sterile distilled water and aliquot to avoid repeated freezing and thawing. Stable for 6 months at -80 centigrade after reconstitution.
Reconstitute with distilled water prior to usage.