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Home > Products > Recombinant Proteins > Recombinant Human HIST3H3 protein, His-tagged

Recombinant Human HIST3H3 protein, His-tagged

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"HIST3H3" Related Products.
By Species
Human(9)
By Source
By Tag
His(2)
Cat.No.:
HIST3H3-290H
Product Overview:
Recombinant Human HIST3H3(2-137 aa) fused with His tag at N-terminal was expressed in E. coli.
Description:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Source:
E. coli
Species:
Human
Tag:
His
Form:
Formulated in 10 mM PBS, pH 7.4, 130 mM NaCl, 10% glycerol and 1 mM DTT.
Molecular Mass:
15.4 kDa
Purity:
> 80% (SDS-PAGE)
Applications:
Suitable substrate for histone methyltransferases
Storage:
Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot protein into single use aliquots. Store remaining undiluted protein in aliquots at -70 centigrade. Note: Protein is very sensitive to freeze/thaw cycles.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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