Recombinant Human HMOX1(Met1-Thr261) fused with His tag at C-terminal was expressed in E. coli.
Predicted N Terminal:
Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Measured by its ability to oxidize hemin to biliverdin. The specific activity is >5 pmol/min/μg, as measured under the described conditions.
Predicted Molecular Mass: 31 kDa;SDS-PAGE: 29-31 kDa, reducing conditions.
<1.0 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 centigrade as supplied.3 months, -20 to -70 centigrade under sterile conditions after opening.