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Recombinant Human HNMT protein, His-tagged

Cat.No.: HNMT-22H
Product Overview: Recombinant Human HNMT(Ala2-Ala292) fused with an N-terminal HM and 6-His tag was expressed in E. coli.
Source: E. coli
Species: Human
Tag: His
Predicted N Terminal: His (of HM) and Ser7
Form: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Bio-activity: Measured by its ability to methylate histamine. The specific activity is >425 pmol/min/μg.
Molecular Mass: Predicted Molecular Mass: 34 kDa;SDS-PAGE: 32-36 kDa, reducing conditions.
Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.
Gene Name: HNMT histamine N-methyltransferase [ Homo sapiens ]
Official Symbol: HNMT
Synonyms: HNMT; histamine N-methyltransferase; HMT; HNMT-S1; HNMT-S2;
Gene ID: 3176
mRNA Refseq: NM_001024074
Protein Refseq: NP_001019245
MIM: 605238
UniProt ID: P50135
Chromosome Location: 2q22.1
Pathway: Histidine metabolism, organism-specific biosystem; Histidine metabolism, conserved biosystem; histamine degradation, organism-specific biosystem; histamine degradation, conserved biosystem;
Function: histamine N-methyltransferase activity; methyltransferase activity; transferase activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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