Recombinant Human HNMT(Ala2-Ala292) fused with an N-terminal HM and 6-His tag was expressed in E. coli.
Predicted N Terminal:
His (of HM) and Ser7
Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Measured by its ability to methylate histamine. The specific activity is >425 pmol/min/μg.
Predicted Molecular Mass: 34 kDa;SDS-PAGE: 32-36 kDa, reducing conditions.
<0.10 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.