Human ICOS / CD278 Protein, Fc Tag, Glu 21 - Phe 141 (Accession # NP_036224) was produced in human 293 cells (HEK293).
Predicted N Terminal:
Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% Trehalose.
Measured by its binding ability in a functional ELISA against immobilized Human B7-H2, His Tag (0.1μg/well). The linear is 0.07-2.3 ng/mL.
Human ICOS, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 40.4 kDa. The reducing protein migrates as 45-50 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
50% human ICOS (Fc Tag) and 50% human IgG1 Fc
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.