Recombinant Human IDS Protein, His-tagged
Recombinant human IDS precursor (NP_000193.1) (Met 1-Pro 550) was expressed with a C-terminal polyhistidine tag.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).The specific activity is >1.0 pmoles/min/μg.
The secreted recombinant human IDS with the propeptide consists of 536 amino acids after removal of the signal peptide and has a predicted molecular mass of 61 kDa. In SDS-PAGE under reducing conditions, it migrates as several bands with apparent molecular mass between 85-95 KDa due to glycosylation.
< 1.0 EU per μg of the protein as determined by the LAL method.
>87 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.