Recombinant human IGFBP4 cDNA (22-258aa, derived from BC016041) fused with T7-His-TEV cleavage site Tag (29aa) at N-terminal was expressed in E. coli.
0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
MASMTGGQQMGRGHHHHHHGNLYFQGGEFDEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPC GVYTPRCGSGLRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRRCLQKH FAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQSRTHEDLYIIPIPNCDRNGNFHPKQCHP ALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFRE
1. May be used for in vitro IGFBP4 mediated VEGF pathway regulation study on endothelial cell growth with this protein as either coating matrix protein or as soluble factor.
Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.