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Active Recombinant Human IGHG1 protein, His & Avi-tagged, Biotinylated

Cat.No. : IGHG1-372H
Product Overview : Biotinylated polyhistidine tagged human IgG1 Fc is expressed from human HEK293 cells. It contains AA Pro 100 - Lys 330 (Accession # P01857(C103S)). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
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Description : Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
Source : HEK293
Species : Human
Tag : His & Avi
Predicted N Terminal : Ala
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Bio-activity : Immobilized Recombinant Protein A at 2 μg/mL (100 µl/well),can bind Biotinylated Human IgG1 Fc protein with a linear range of 0.8-12.5 ng/mL.
Molecular Mass : This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 28.8 kDa. The protein migrates as 36 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 60-66 kDa under non-reducing (NR) condition.
Protein length : Pro 100 - Lys 330
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Storage buffer : 50 mM Tris, 100 mM Glycine, pH7.5
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Shipping : Powder,RT
Gene Name : IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) [ Homo sapiens (human) ]
Official Symbol : IGHG1
Synonyms : constant region of heavy chain of IgG1; immunoglobulin gamma 1 (Gm marker)
Gene ID : 3500

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Does IGHG1 protein have an effect on the activity of immune cells? 08/19/2022

Yes, IGHG1 protein regulates the activity and function of immune cells by interacting with their receptors.

What disease markers can IGHG1 protein be used as a marker in clinical practice? 08/09/2021

IGHG1 protein is expressed at abnormal levels in some immune diseases and can be detected as one of the markers of diseases, such as rheumatoid arthritis.

What is the mechanism of action of IGHG1 protein in humoral immunity? 07/29/2021

IGHG1 protein is involved in humoral immunity, protecting the body from infection by interacting with pathogens, neutralizing toxins, and assisting in the phagocytosis of immune cells.

How is IGHG1 protein related to immunotherapy response? 11/20/2020

Yes, there may be a correlation between IGHG1 protein and response to immunotherapy, and researchers are exploring it as a predictor of immunotherapy.

Is IGHG1 protein associated with antibody-dependent cytotoxicity (ADCC)? 09/13/2020

Yes, the IGHG1 protein is an important component involved in antibody-dependent cytotoxicity (ADCC) and enhances cytotoxicity by interacting with effector cells.

Is the expression level of IGHG1 protein related to the severity of the disease in immune diseases? 02/04/2020

Yes, some studies have found that the expression level of IGHG1 protein is related to the severity of immune diseases, which may be one of the indicators to evaluate disease progression and prognosis.

Customer Reviews (3)

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Reviews
09/23/2022

    Good stability, suitable for long-term storage.

    11/17/2021

      It can induce specific immune response accurately and stably, which is of great application value.

      05/20/2021

        Fluorescent labeling works very well, it helps with imaging studies, it works very well.

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