Recombinant human IgG2 Fc region (P01859) (Glu 99-Lys 327, 257Ser/Ala) was expressed and purified.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its ability to bind human CD32a-His (Cat:10374-H08H1) in a functional ELISA.
The recombinant human IgG2-Fc consists of 228 amino acids and has a predicted molecular mass of 25 kDa. As a result of glycosylation, the apparent molecular mass of IgG2-Fc is approximately 34 kDa in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.