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Home > Products > Recombinant Proteins > Recombinant Human IGHG2 Protein

Recombinant Human IGHG2 Protein

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"IGHG2" Related Products.
By Species
Human(1)
By Source
HEK293(1)
Cat.No.:
IGHG2-548H
Product Overview:
Recombinant human IgG2 Fc region (P01859) (Glu 99-Lys 327, 257Ser/Ala) was expressed and purified.
Source:
HEK293
Species:
Human
Predicted N Terminal:
Glu 99
Form:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity:
Measured by its ability to bind human CD32a-His (Cat:10374-H08H1) in a functional ELISA.
Molecular Mass:
The recombinant human IgG2-Fc consists of 228 amino acids and has a predicted molecular mass of 25 kDa. As a result of glycosylation, the apparent molecular mass of IgG2-Fc is approximately 34 kDa in SDS-PAGE under reducing conditions.
Endotoxin:
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity:
>90 % as determined by SDS-PAGE.
Stability:
Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage:
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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