Recombinant Human IL10RA, transcript variant 1, fused with MYC/DDK tag at C-terminal was expressed in HEK293.
The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene.
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Predicted Molecular Mass: 150.4 (monomer) & 55.9 (truncated) kDa;SDS-PAGE: 210-225 kDa, reducing conditions.
> 80% as determined by SDS-PAGE and Coomassie blue staining
>50 ug/mL as determined by microplate BCA method