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Recombinant Human IL12A & IL12B Protein, His-tagged

"IL12A/IL12B" Related Products.
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IL12A & IL12B-1242H
Product Name:
Recombinant Human IL12A & IL12B Protein, His-tagged
Product Overview:
Recombinant p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; Recombinant p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) w
Predicted N Terminal:
Arg 23 & Ile 23
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. 2. Measured in a cell proliferation assay using Anti-CD28 and Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 0.1-0.6 ng/mL.
Molecular Mass:
The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 40 & 43 kDa respectively in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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