Recombinant Human IL12A/IL27B protein, Fc-tagged
Recombinant Human IL27B subunit(Met 1-Lys 229) and the mature form of human IL12 p35 subunit(Arg 23-Ser 219) linked by a polypeptide linker was fused with the Fc region of human IgG1 at the C-terminus was expressed in HEK293.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4
The recombinant human IL35/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 645 amino acids and has a predicted molecular mass of 73.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL35/Fc monomer is approximately 90-100 kDa due to glycosylation.
< 1.0 EU per μg of the protein as determined by the LAL method
> 85 % as determined by SDS-PAGE
Samples are stable for up to twelve months from date of receipt at -70 centigrade
Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.