Recombinant extracellular domain of human IL13Rα2 (NP_000631.1) (Met1-Leu342) was expressed with the a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its ability to inhibit IL13 dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.02-0.08 μg/mL.
The recombinant human IL13Rα2 consists of 325 amino acids and predicts a molecular mass of 38 KDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.