Recombinant Human IL15(Asn 49 - Ser 162) was expressed in HEK293.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse CTLL-2 cells.The ED50 < 0.5 ng/mL, corresponding to a specific activity of >2X10^6 Unit/mg.
Has a calculated MW of 12.8 kDa. The predicted N-terminus is Asn 49. DTT-reduced Protein migrates as 13 kDa in SDS-PAGE.
Less than 1.0 EU per μg of the ActiveMax® Human IL-15 by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).