Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human IL1A protein, His & T7-tagged

Cat.No. : IL1A-625H
Product Overview : Recombinant Human IL1A aa. (Ser113~Ala271 (Accession # Q53QF9)) fused with N-terminal His & T7 tag was produced in E. coli cells.
  • Specification
  • Gene Information
  • Related Products
Description : The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
Source : E. coli
Species : Human
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 21.6kDa
Protein length : Ser113~Ala271 (Accession # Q53QF9)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 6.5.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name : IL1A interleukin 1 alpha [ Homo sapiens (human) ]
Official Symbol : IL1A
Synonyms : IL1A; interleukin 1 alpha; IL1; IL-1A; IL1F1; IL1-ALPHA; interleukin-1 alpha; IL-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha
Gene ID : 3552
mRNA Refseq : NM_000575.4
Protein Refseq : NP_000566.3
UniProt ID : Q53QF9

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (10)

Ask a question
What methods are employed to dissect the intricate cross-talk between IL-1α and other cytokines, shedding light on its role in immune modulation? 11/02/2021

Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.

What experimental approaches allow researchers to dissect the paradoxical roles of IL-1α as both a pro-inflammatory and tissue repair mediator in distinct contexts? 09/22/2021

IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.

How are cutting-edge techniques like single-cell RNA sequencing utilized to unravel the heterogeneity in IL-1α-responsive cell populations and their functional implications? 06/07/2021

Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.

How is the intricate web of IL-1α's pro-inflammatory signaling pathways decoded using advanced experimental techniques? 05/26/2021

IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.

How are state-of-the-art genetic editing tools such as CRISPR-Cas9 applied to unravel IL-1α's precise contributions to inflammatory diseases? 04/06/2021

Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.

How do researchers manipulate in vitro co-culture systems to dissect the dynamic interplay between immune cells and stromal cells in IL-1α-mediated responses? 06/18/2020

In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.

What experimental strategies enable the identification of specific cellular receptors and downstream effectors responsible for IL-1α's diverse physiological effects? 03/19/2020

Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.

How are sophisticated imaging techniques like live-cell microscopy harnessed to capture the real-time dynamics of IL-1α release and its impact on neighboring cells? 12/02/2019

Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.

How is the quantitative relationship between IL-1α levels and disease progression established through longitudinal studies and statistical modeling? 07/15/2018

Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.

How do multi-omics analyses, including transcriptomics and proteomics, provide a holistic view of the network of molecules influenced by IL-1α signaling? 06/04/2018

Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.

Customer Reviews (3)

Write a review
Reviews
04/08/2021

    High solubility.

    07/02/2020

      Good for intracellular staining.

      03/13/2019

        Effective in apoptosis assays.

        Ask a Question for All IL1A Products

        Required fields are marked with *

        My Review for All IL1A Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends