Recombinant Human IL4 Protein

Cat.No.: IL4-120H
Product Overview: Recombinant Human IL-4, His 25 - Ser 153 (Accession # AAH67514) was produced in human 293 cells (HEK293).
Source: HEK293
Species: Human
Predicted N Terminal: His 25
Form: Lyophilized from sterile 20 mM MES, 200 mM NaCl, pH6.0, 10% Trehalose.
Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human IL-4 at 5 μg/mL (100 μL/well) can bind Human IL-4 R alpha, His Tag with a linear range of 0.01-0.1 μg/mL. The bio-activity was determined by dose-dependent stimulation of the proliferation of TF-1 cells. The ED50< 0.2 ng/mL, corresponding to a specific activity of >5000000 Unit/mg.
Molecular Mass: Human IL-4 contains no "tag", and has a calculated MW of 14.9 kDa. The reducing protein migrates as 15 and 19 kDa in SDS-PAGE due to glycosylation.
Endotoxin: Less than 0.1 EU per μg by the LAL method.
Purity: >90% as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: IL4 interleukin 4 [ Homo sapiens ]
Official Symbol: IL4
Synonyms: IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte stimulatory factor 1; MGC79402; binetrakin; pitrakinra; IL-4; BSF-1; BCGF-1;
Gene ID: 3565
mRNA Refseq: NM_000589
Protein Refseq: NP_000580
MIM: 147780
UniProt ID: P05112
Chromosome Location: 5q23-q31
Pathway: Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD40/CD40L signaling, organism-specific biosystem;
Function: cytokine activity; growth factor activity; interleukin-4 receptor binding; protein binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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