Recombinant Human IL5 protein
Recombinant Human IL5 was expressed in Insect cells.
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
The biological activity of Human IL-5 was determined in a cell proliferation assay using a factor-dependent human cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 334). The expected ED50 for this effect is typically 0.1 - 0.2 ng/ml.
The predicted molecular weight of Recombinant Human IL-5 is Mr 13.1 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 13-15 kDa.
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<1.0 EU/µg as determined by the LAL method
>97% by SDS-PAGE and analyzed by silver stain.
Working aliquots of this recombinant protein solution are stable for up to twelve months at -20 centigrade to -70 centigrade in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2 centigrade - 8 centigrade for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20 centigrade to -70 centigrade in a manual defrost freezer. Avoid repeated freeze thaw cycles.