Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Human IL5 protein

Recombinant Human IL5 protein

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Cat.No.:
IL5-241H
Product Overview:
Recombinant Human IL5 was expressed in Insect cells.
Source:
Insect cells
Species:
Human
Form:
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Bio-activity:
The biological activity of Human IL-5 was determined in a cell proliferation assay using a factor-dependent human cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 334). The expected ED50 for this effect is typically 0.1 - 0.2 ng/ml.
Molecular Mass:
The predicted molecular weight of Recombinant Human IL-5 is Mr 13.1 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 13-15 kDa.
AA Sequence:
i pteiptsalv ketlallsth rtllianetl ripvpvhknh qlcteeifqg igtlesqtvq ggtverlfkn lslikkyidg qkkkcgeerr rvnqfldylq eflgvmntew iies
Endotoxin:
<1.0 EU/µg as determined by the LAL method
Purity:
>97% by SDS-PAGE and analyzed by silver stain.
Storage:
Working aliquots of this recombinant protein solution are stable for up to twelve months at -20 centigrade to -70 centigrade in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2 centigrade - 8 centigrade for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20 centigrade to -70 centigrade in a manual defrost freezer. Avoid repeated freeze thaw cycles.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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