Recombinant Human IL5 was expressed in Insect cells.
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
The biological activity of Human IL-5 was determined in a cell proliferation assay using a factor-dependent human cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 334). The expected ED50 for this effect is typically 0.1 - 0.2 ng/ml.
The predicted molecular weight of Recombinant Human IL-5 is Mr 13.1 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 13-15 kDa.
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<1.0 EU/µg as determined by the LAL method
>97% by SDS-PAGE and analyzed by silver stain.
Working aliquots of this recombinant protein solution are stable for up to twelve months at -20 centigrade to -70 centigrade in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2 centigrade - 8 centigrade for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20 centigrade to -70 centigrade in a manual defrost freezer. Avoid repeated freeze thaw cycles.