Recombinant Human IL5 protein

• Cat.No.: IL5-144H
• Product Overview: Recombinant Human IL5 was expressed in E. coli.

Specification

• Description: Interleukin 5 (IL5) is a specific hematopoietic growth factor that is responsible for the growth and differentiation of eosinophils. IL5 promotes the growth of immature hematopoietic progenitor cells BFU-E while it causes differentiation of CFU-E the proliferation of which is inhibited by IL5. B cells can be made responsive to IL5 by treatment with suboptimal doses of IL1. Interleukin 5 also promotes the generation of cytotoxic T cells from thymocytes. In thymocytes IL5 induces the expression of high affinity IL2 receptors. Human and mouse IL5 is cross-species reactive. Recombinant Human IL5 is a 26.5 kDa disulfide-linked homodimerized protein.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in Tris with NaCl
• Molecular Mass: 13.0 kDa
• Endotoxin: <1.0 EU/μg of recombinant protein as determined by the LAL method.
• Purity: >95%
• Applications: Cell Assay
• Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 centigrade. Upon reconstitution, this cytokine can be stored in working aliquots at 2 centigrade - 8 centigrade for one month, or at -20 centigrade for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Gene Information

• Gene Name: IL5 interleukin 5 (colony-stimulating factor, eosinophil) [ Homo sapiens ]
• Official Symbol: IL5
• Synonyms: IL5; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; B cell differentiation factor I; EDF; eosinophil differentiation factor; IL 5; interleukin 5; T cell replacing factor; TRF; T-cell replacing factor; B-cell differentiation factor I; IL-5
• Gene ID: 3567
• mRNA Refseq: NM_000879
• Protein Refseq: NP_000870
• MIM: 147850
• UniProt ID: P05113
• Chromosome Location: 5q23-q31
• Pathway: Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem
• Function: cytokine activity; growth factor activity; interleukin-5 receptor binding; protein binding