| Product overview : |
Recombinant Human IL5 (P05113) aa. (IIe20~Ser134) fused with His tag was produced in HEK293F cells. |
| Description : |
This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. |
| Source : |
HEK293F |
| Species : |
Human |
| Tag : |
His |
| Form : |
Freeze-dried powder |
| Molecular Mass : |
Predicted Molecular Mass: 14.8kDa; Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions. |
| Protein length : |
IIe20~Ser134 |
| Endotoxin : |
<1.0EU per 1ug (determined by the LAL method) |
| Purity : |
> 97% |
| Characteristic : |
Isoelectric point is 7. |
| Applications : |
SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling |
| Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
| Concentration : |
200ug/mL |
| Storage buffer : |
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300. |
| Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex |
