Recombinant mature form of human IL6 (NP_000591.1) (Val 30-Met 212) was expressed, with an initial Met at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 μg/ml (100 μl/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/mL.2. Measured in a cell proliferation assay using TF-1 human erythr
The recombinant human IL6 consists of 184 amino acids and migrates with an apparent molecular mass of 20.3 kDa as estimated in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.