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Recombinant Human IRS1 HEK293T cell lysate

Cat.No.: IRS1-12HCL
Product Overview: Human IRS1 was derived in Human HEK293T cell line. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate.
Description: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
Source: HEK293
Species: Human
Tag: Myc/DDK
Form: The cell lysate is provided in RIPA buffer.
Molecular Mass: 131.4 kDa
Storage: Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name: IRS1 insulin receptor substrate 1 [ Homo sapiens ]
Official Symbol: IRS1
Synonyms: IRS1; insulin receptor substrate 1; HIRS 1; IRS-1; HIRS-1;
Gene ID: 3667
mRNA Refseq: NM_005544
Protein Refseq: NP_005535
MIM: 147545
UniProt ID: P35568
Chromosome Location: 2q36

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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