Recombinant Human IST1, fused with N-His tag, was expressed in E. coli.
Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
20mM Tris.Cl, 50mM NaCl, 50% Glycerol, pH9.0.
38.5kDa(including fusion tag)
350aa(including fusion tag)
WB, ELISA, IP, antibody production, protein array
Store at -80°C and avoid repeated freeze-thaw cycles