Recombinant Human ITGAV(Phe 31 - Val 992)/ITGB1 (Gly 27 - Asp 718) Heterodimer Protein, Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB1 contains no tag but a basic tail at the C-terminus, was expressed in HEK293.
Integrin alpha-5/beta-1 is a receptor for ibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-V/beta-1 is also a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition.
Predicted N Terminal:
The predicted N-terminus is Phe 31 (ITGAV) & Gln 21 (ITGB1)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Has a calculated MW of 113 kDa (ITGAV) and 83.7 kDa (ITGB1), The reducing (R) protein migrates as 145 kDa (ITGAV) and 116 kDa (ITGB6) respectively due to glycosylation.
ITGAV(Phe 31 - Val 992)/ITGB3 (Gly 27 - Asp 718)
Less than 1.0 EU per μg of the Human ITGAV & ITGB1 Heterodimer Protein by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).