Active Recombinant Human ITGAV&ITGB8 protein, His-tagged
Cat.No. : | ITGAV&ITGB1-745H |
Product Overview : | Recombinant Human ITGAV&ITGB8 protein(Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8) ), fused with His tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag with a linear range of 1-31 ng/mL.Immobilized Human Fibronectin at 10 μg/mL (100 μL/well) can bind Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.313-10 μg/mL.Immobilized Human Vitronectin at 5 μg/mL (100 μL/well) can bind Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.313-10 μg/mL. |
Molecular Mass : | Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 112.9 kDa (ITGAV) and 76.5 kDa (ITGB8). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGAV) and 80-90 kDa (ITGB8) respectively due to glycosylation. |
Proteinlength : | Phe31-Val992(ITGAV)&Glu43-Arg684(ITGB8) |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | ITGAV integrin, alpha V [ Homo sapiens ] |
Official Symbol : | ITGAV |
Synonyms : | ITGAV; integrin, alpha V; antigen identified by monoclonal L230 , integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) , MSK8, vitronectin receptor , VNRA, VTNR; integrin alpha-V; CD51; integrin alphaVbeta3; vitronectin receptor subunit alpha; antigen identified by monoclonal L230; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; VNRA; VTNR; DKFZp686A08142; |
Gene ID : | 3685 |
mRNA Refseq : | NM_001144999 |
Protein Refseq : | NP_001138471 |
MIM : | 193210 |
UniProt ID : | P06756 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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