Recombinant Human ITGAV(Phe 31 - Val 992)/ITGB6 (Gly 22 - Asn 707) Heterodimer Protein, Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB6 contains no tag but a basic tail at the C-terminus, was expressed in HEK293.
Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.
Predicted N Terminal:
The predicted N-terminus is Phe 31 (ITGAV) & Gly 22 (ITGB6)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Has a calculated MW of 113 kDa (ITGAV) and 79.6 kDa (ITGB6). The reducing (R) protein migrates as 145 kDa (ITGAV) and 115 kDa (ITGB6) respectively due to glycosylation.
Phe 31 - Val 992 (ITGAV) & Gly 22 - Asn 707 (ITGB6) (Accession # NP_002201 (ITGAV)& AAI21179 (ITGB6))
Less than 1.0 EU per μg of the Human ITGAV & ITGB6 Heterodimer Protein by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).