Active Recombinant Human KDM1A protein

• Cat.No.: KDM1A-48H
• Product Overview: Recombinant Human KDM1A(158–end) was expressed in E. coli.

Specification

• Description: This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 1 mM DTT, 1 mM EDTA, 0.05% Tween-20, and 20% glycerol.
• Bio-activity: ≥20.6 pmol/min/µg
• Molecular Mass: 77.9 kDa
• AA Sequence: GPLGSPEFAPPEEENESEPEEPSGV EGAAFQSRLPHDRMTSQEAACFPDI ISGPQQTQKVFLFIRNRTLQLWLDN PKIQLTFEATLQQLEAPYNSDTVLV HRVHSYLERHGLINFGIYKRIKPLP TKKTGKVIIIGSGVSGLAAARQLQ SFGMDVTLLEARDRVGGRVATFRKG NYVADLGAMVVTGLGGNPMAVVSKQ VNMELAKIKQKCPLYEANGQAVPKE KDEMVEQEFNRLLEATSYLSHQLDF NVLNNKPVSLGQALEVVIQLQEKHV KDEQIEHWKKIVKTQEELKELLNK MVNLKEKIKELHQQYKEASEVKPPR DITAEFLVKSKHRDLTALCKEYDEL AETQGKLEEKLQELEANPPSDVYL SSRDRQILDWHFANLEFANATPLST LSLKHWDQDDDFEFTGSHLTVRNGY SCVPVALAEGLDIKLNTAVRQVRYT ASGCEVIAVNTRSTSQTFIYKCDAV LCTLPLGVLKQQPPAVQFVPPLPEW KTSAVQRMGFGNLNKVVLCFDRVF WDPSVNLFGHVGSTTASRGELFLFW NLYKAPILLALVAGEAAGIMENISD DVIVGRCLAILKGIFGSSAVPQPK ETVVSRWRADPWARGSYSYVAAGSS GNDYDLMAQPITPGPSIPGAPQPIP RLFFAGEHTIRNYPATVHGALLSGL REAGRIADQFLGAMYTLPRQATPGV PAQQSPSM
• Purity: ≥90%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: >6 months at –80 centigrade.
• Concentration: 0.3 mg/ml

Gene Information

• Gene Name: KDM1A lysine (K)-specific demethylase 1A [ Homo sapiens ]
• Official Symbol: KDM1A
• Synonyms: KDM1A; lysine (K)-specific demethylase 1A; amine oxidase (flavin containing) domain 2 , AOF2, KDM1, lysine (K) specific demethylase 1; lysine-specific histone demethylase 1A; BHC110; KIAA0601; LSD1; lysine (K)-specific demethylase 1; BRAF35-HDAC complex protein BHC110; lysine-specific histone demethylase 1; amine oxidase (flavin containing) domain 2; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; flavin-containing amine oxidase domain-containing protein 2; AOF2; KDM1
• Gene ID: 23028
• mRNA Refseq: NM_015013
• Protein Refseq: NP_055828
• MIM: 609132
• UniProt ID: O60341
• Chromosome Location: 1p36.12
• Pathway: Coregulation of Androgen receptor activity, organism-specific biosystem; Factors involved in megakaryocyte development and platelet production, organism-specific biosystem; Hemostasis, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem
• Function: MRF binding; androgen receptor binding; chromatin binding; demethylase activity; enzyme binding; flavin adenine dinucleotide binding; histone demethylase activity; histone demethylase activity (H3-K4 specific); histone demethylase activity (H3-K9 specific); histone demethylase activity (H3-dimethyl-K4 specific); ligand-dependent nuclear receptor transcription coactivator activity; oxidoreductase activity; p53 binding; protein binding; transcription factor binding; transcription regulatory region DNA binding