Recombinant Human L3MBTL1(190-530 aa)(3XMBT DOMAIN) fused with GST tag at N-terminal was expressed in E. coli.
L3MBTL1 3xMBT contains the three MBT repeat domains present in the protein. L3MBTL1 is a member of the Polycomb group of proteins and binds to broad range of proteins that exhibit mono- and dimethylation, including histone H1, histone H4, p53 and Rb. The truncated version of L3MBTL1 present in the 3xMBT domain protein serves as a useful tool for the detection of mono- and dimethylation of a wide variety of proteins.
In 20 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM DTT, 20% glycerol.
L3MBTL1 3xMBT Domain, Recombinant Human is useful for the detection of protein mono- and dimethylation in far-Western assays, for the immunoprecipitation of methylated proteins in pull-down experiments, or as a readout of methyltransferase enzyme activity.
Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.