Recombinant Human LCAT Protein, His-tagged

• Cat.No.: LCAT-526H
• Product Overview: Recombinant human LCAT (NP_000220.1) (Met1-Glu440) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Phe 25
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant human LCAT consists 427 amino acids and predicts a molecular mass of 48.5 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: LCAT lecithin-cholesterol acyltransferase [ Homo sapiens ]
• Official Symbol: LCAT
• Synonyms: LCAT; lecithin-cholesterol acyltransferase; phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase
• Gene ID: 3931
• mRNA Refseq: NM_000229
• Protein Refseq: NP_000220
• MIM: 606967
• UniProt ID: P04180
• Chromosome Location: 16q22.1
• Pathway: Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; HDL-mediated lipid transport, organism-specific biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Lipoprotein metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem