Recombinant Human LCMT1 fused with GST tag at N-terminal was expressed in Insect cells.
Leucine carboxyl methyltransferase 1 (LCMT1) exclusively methylates the carboxyl group of leucine-309 in the catalytic subunit of protein phosphatase 2A. LCMT1 is essential for normal progression through mitosis and cell survival. Knocking-down LCMT1 leads to reduced formation of PP2A heterodimers and increased apoptosis. LCMT1 also regulates the association of PP2A and its substrate, Tau protein, with plasma membrane in neuroblastoma cells. Decreased level of PP2A methylation is found in Alzheimer disease.
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
1 year at -70 centigrade from the date of shipment
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.