Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Human LIF protein, His-tagged

Recombinant Human LIF protein, His-tagged

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By Species
Human(22)
Mouse(20)
Rat(4)
By Tag
Fc(4)
GST(2)
His(7)
His-GST(1)
N/A(4)
StrepII(1)
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Cat.No.:
LIF-134H
Product Overview:
Recombinant Human LIF(23-202aa) fused with His tag at C-terminal was expressed in Insect cells.
Description:
LIF, as known as leukemia inhibitory factor, is a pleiotropic glycoprotein belonging to the LI6 family of cytokines. This protein is involved in growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, embryogenesis and inflammation. It is produced by the adrenal cortex and likely enhances its production of cortisol and aldosterone. Also, it can function as an autocrine growth factor in some pancreatic cancers, but induced differentiation in the leukemic cell line M1.
Source:
Insect cells
Species:
Human
Tag :
His
Form:
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Bio-activity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is less or equal to 0.5 ng/ml.
Molecular Mass:
20.8kDa (189aa)
18-40kDa (SDS-PAGE under reducing conditions.)
AA Sequence:
ADPSPLPITP VNATCAIRHP CHNNLMNQIR SQLAQLNGSA NALFILYYTA QGEPFPNNLD KLCGPNVTDF PPFHANGTEK AKLVELYRIV VYLGTSLGNI TRDQKILNPS ALSLHSKLNA TADILRGLLS NVLCRLCSKY HVGHVDVTYG PDTSGKDVFQ KKKLGCQLLG KYKQIIAVLA
QAFHHHHHH
Endotoxin:
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity:
> 90% by SDS - PAGE
Applications:
SDS-PAGE
Storage:
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration:
0.25mg/ml (determined by Absorbance at 280nm)


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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