Recombinant Human LIF(23-202aa) fused with His tag at C-terminal was expressed in Insect cells.
LIF, as known as leukemia inhibitory factor, is a pleiotropic glycoprotein belonging to the LI6 family of cytokines. This protein is involved in growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, embryogenesis and inflammation. It is produced by the adrenal cortex and likely enhances its production of cortisol and aldosterone. Also, it can function as an autocrine growth factor in some pancreatic cancers, but induced differentiation in the leukemic cell line M1.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is less or equal to 0.5 ng/ml.
18-40kDa (SDS-PAGE under reducing conditions.)
ADPSPLPITP VNATCAIRHP CHNNLMNQIR SQLAQLNGSA NALFILYYTA QGEPFPNNLD KLCGPNVTDF PPFHANGTEK AKLVELYRIV VYLGTSLGNI TRDQKILNPS ALSLHSKLNA TADILRGLLS NVLCRLCSKY HVGHVDVTYG PDTSGKDVFQ KKKLGCQLLG KYKQIIAVLA
< 1.0 EU per 1 microgram of protein (determined by LAL method)
> 90% by SDS - PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.25mg/ml (determined by Absorbance at 280nm)