Recombinant Human LILRB4(Gln 22 - Glu 259) fused with Fc fragment of human IgG1 at the C-terminus was expressed in HEK293.
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Has a calculated MW of 52.9 kDa. The predicted N-terminus is Gln 22. DTT-reduced Protein migrates as 53-66 kDa due to glycosylation.
Less than 1.0 EU per μg of the rhLILRB4 Fc Chimera by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).