Recombinant Human LYN Protein, GST-tagged
Recombinant human LYN isoform a (NP_002341.1) (Met 1-Pro 512) was fused with the GST tag at the N-terminus.
Predicted N Terminal:
Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM Reduced Glutathione, 10% gly, 0.5mM PMSF.
The specific activity was determined to be 30 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate.
The recombinant human LYN/GST chimera consists of 736 amino acids and predicts a molecular mass of 84.8 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>94 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.