Active Recombinant Human MADCAM1 protein, His-tagged

• Cat.No.: MADCAM1-3982H
• Product Overview: Recombinant Human MADCAM1 protein(Gln 19 - Gln 317), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Human MAdCAM-1, His Tag immobilized on CM5 Chip can bind Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free with an affinity constant of 0.227 μM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 33.3 kDa. The protein migrates as 46-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Gln 19 - Gln 317
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MADCAM1 mucosal vascular addressin cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: MADCAM1
• Synonyms: MADCAM1; mucosal vascular addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule 1; MACAM1; MAdCAM-1; hMAdCAM-1; mucosal addressin cell adhesion molecule-1
• Gene ID: 8174
• mRNA Refseq: NM_130760
• Protein Refseq: NP_570116
• MIM: 102670
• UniProt ID: Q13477