Recombinant Human p38α [9-352 amino acids of accession number NP_620581.1] was co-expressed as N-terminal DYKDDDDK tagged, biotinylated protein (43 kDa) with human His-MAP2K6[1-334(end) amino acids of accession number NP_002749.2] and His-MLK3[99-398 ami
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
>90% as determined by SDS-PAGE and CBB staining
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.