Recombinant Human MAX, transcript variant 1, fused with His tag at C-terminal was expressed in E. coli.
The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Mutations of this gene have been reported to be associated with hereditary pheochromocytoma. A pseudogene of this gene is located on the long arm of chromosome 7. Alternative splicing results in multiple transcript variants.
Lyophilized from a 0.2 µM filtered solution of 20mM Tris-HCl, 50mM Imidazole, 250mM NaCl, pH 8.5. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
< 0.1 EU per µg protein as determined by LAL test
>95% as determined by SDS-PAGE and Coomassie blue staining
Resuspend the protein to the desired concentration in proper buffer.