Recombinant Human MLLT3(1-149 aa)(YEATS DOMAIN, F78A) fused with GST tag at N-terminal was expressed in E. coli.
The AF9 protein is part of the super elongation complex and also associates with the histone methyltransferase DOT1L. Translocation of the AF9 gene to the MLL gene, resulting in an MLL/AF9 fusion is the most common chromosomal rearrangement involving MLL in de novo AML. The YEATS domain of binds strongly to histone H3K9 acetylation and, to a lesser extent, H3K27 and H3K18 acetylation. AF9 YEATS Domain, F78A, Recombinant Human contains a phenylalanine to alanine substitution at position 78.
Recombinant protein at 1 mg/ml in 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1mM EDTA, 1 mM DTT, 20% glycerol.
AF9 YEATS Domain, F78A, Recombinant Human is useful for protein binding and screening experiments using AF9 YEATS Domain, Recombinant Human to examine acetylated protein substrates.
Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.